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‪Cecilia Magnusson‬ - ‪Google Scholar‬

The plasmapheresis platform is currently being integrated with cancer bio-marker microarrays for on-line multiplex immunoassaying, including secondary detector antibody incubation. Acoustophoresis has also been developed to purify/extract components from complex matrices using affinity probe acti-vated microbeads, i.e. Affinity Acoustophoresis. Acoustophoresis. Our approach to separate CTCs from white blood cells is using a label-free ultrasound standing wave-based microfluidics platform called Acoustophoresis (Magnusson et al 2017, Antfolk et al 2015, Augustsson et al 2012). This cell separation is based on intrinsic cellular properties such as size, density and compressibility.

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abstract = "Metastatic disease is responsible for most cancer deaths, and hematogenous spread through circulating tumor cells (CTC) is a prerequisite for tumor  using high throughput multiplex acoustophoresis, Lab Chip, 2019, 19, cancer cells by means of acoustophoresis, Integrative Biology, 2016,  Deierborg, T.; Label-free concentration of viable neurons, hESCs and cancer acoustophoresis chip enabling tumor cell enrichment from white blood cells. The impact could be huge for the millions of people living with cancer or “Microfluidic acoustic separation (or acoustophoresis) is gentle on  We demonstrate an acoustophoresis method for size-based separation, we demonstrate an on-chip fluorescence assay of the isolated cancer cells by  Free flow acoustophoresis: microfluidic-based mode of particle and cell Microfluidic, label-free enrichment of prostate cancer cells in blood based on  Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis. P Augustsson, C Magnusson, M Nordin, H Lilja, T Laurell. Microparticle Acoustophoresis in Aluminum-Based Acoustofluidic Devices with Cancer cells display acoustic properties enabling acoustophoretic separation  Projekt: Apheresis-acoustophoresis to improve cancer diagnosis in prostate cancer (APACA-PC). Anna Lantz, lnst för Medicinsk Epidemiologi och Biostatistik,  Traditional acoustophoresis cell handling typically displays a lower size limit of about 2 micrometers. However, more recent developments have  This ultrasonic standing wave-based technology, iso-acoustophoresis, offers This enables access to viable and unaltered circulating tumor cells, important for  The Josefsson prostate cancer group is a multi-disciplinary bench-to-bedside research team in the Wallenberg Centre for Molecular Medicine. We aim to  NK cells (orange) attacking an ultrasound-fabricated solid tumor (green) of particles and biological cells (acoustophoresis, acoustic trapping)  in Cytometry and Biomedical Applications: Multidimensional Acoustophoresis Ultrasound-assisted Interactions of Natural Killer Cells with Cancer Cells and  Swedish University dissertations (essays) about ACOUSTOPHORESIS.

However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis | Analytical Chemistry Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers. Surface-marker-based detection of CTC predicts recurrence and survival in colorectal, breast, and prostate cancer.

Lund University Cancer Center Distinguished Seminar

Acoustophoresis is a new lab-on-a-chip technology based on the microfluidic technology that offers a persuasive alternative to both FACS and MACS. label-free microfluidic acoustophoresis can be used to enrich both viable and fixed cancer cells from WBCs with very high recovery and purity. M any types of cancer are diagnosed at the early stages of the disease, enabling treatment with curative intent. However, many patients succumb due to metastases, and the Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis | Analytical Chemistry Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers.

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Here are 10 more facts about prostate cancer. Breast cancer is the second most common cancer found in women — after skin cancer — but that doesn’t mean men aren’t at risk as well. Although the percentage of cases in men is much lower than in women, male breast cancer accounts for a por Whether colon cancer runs in your family or you’re interested in learning about health conditions as part of an effort to improve your well-being, it’s important to understand this type of cancer. According to the American Cancer Society, a Lung cancer is a serious illness which none of us wish to face. Here we look at some of the key symptoms of this disease to watch out for. We also explore how it is diagnosed and the many treatment options now available should you be unfort Lung cancer is a leading type of cancer — and a leading killer — in the United States every year.

Acoustophoresis. Our approach to separate CTCs from white blood cells is using a label-free ultrasound standing wave-based microfluidics platform called Acoustophoresis (Magnusson et al 2017, Antfolk et al 2015, Augustsson et al 2012). This cell separation is based on intrinsic cellular properties such as size, density and compressibility. The aim of this thesis was to compare different ways of dissociating tissue - specifically breast cancer tissue - and sorting out tumour cells and immune cells from the dissociated tissue using acoustophoresis in a microfluidic chip. Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis Forskningsoutput : Tidskriftsbidrag › Artikel i vetenskaplig tidskrift Översikt Using this microchannel configuration, several publications have shown cancer cell lines (breast and prostate) being efficiently separated from WBCs from RBC-lysed blood using acoustophoresis with BASWs [3, 20].This label-free separation occurs because larger-sized cancer cells focus faster to the center pressure node than WBCs in the separation channel; thus, if flow rates are optimized the ACOUSTOPHORESIS -A SOUND APPROACH TO CHIP BASED CELL HANDLING – T. Laurell1,2 1 Dept. Measurement Science and Industrial Electrical Engineering Div. Nanobiotechnology, Lund University, Lund, SWEDEN 2 Dept. Biomedical Engineering, Dongguk University, Seoul, SOUTH KOREA ABSTRACT The basic principles of acoustic cell and particle manipulation in chip integrated acoustic standing … Request PDF | Acoustophoresis in Tumor Cell Enrichment | This chapter outlines the opportunities and current developments of label-free separation of circulating tumor cell (CTC) from blood cells Neither did acoustophoresis affect cell proliferation or the ability of cells to be recultured for several passages ( Figure 4B).
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Our approach to separate CTCs from white blood cells is using a label-free ultrasound standing wave-based microfluidics platform called Acoustophoresis (Magnusson et al 2017, Antfolk et al 2015, Augustsson et al 2012). This cell separation is based on intrinsic cellular properties such as size, density and compressibility.

18 Dec 2019 Making chemotherapy more targeted to the tumour.
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On-chip ultrasonic sample preparation for cell based assays

Anna Lantz, lnst för Medicinsk Epidemiologi och Biostatistik, Karolinska Institutet, får 388 784 kronor. Projekt: Evaluation and optimization of active surveillance for men living with clinically insignificant prostate cancer. Researchers from MIT, Pennsylvania State University, and Carnegie Mellon University have devised a new way to separate cells by exposing them to sound waves They have presented an acoustophoresis device with trifurcation inlet and outlet to separate three different prostate cancer cell lines (DU145, PC3, and LNCaP) from no-cancer control subjects’ blood sample spiked with the mentioned cancer cells . Second part of the thesis focuses on acoustophoresis.

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As a growing corpus of evidence supports that cancer cells exhibit distinct mechanical phenotypes from healthy cells, expanding the method from size to mechanical sensing could represent a pertinent and innovative tool for cancer research. Traditional acoustophoresis cell handling typically displays a lower size limit of about 2 micrometers. However, more recent developments have advanced acoustofluidics to enable access also to bacteria and submicron vesicles where the rising interest in extra cellular vesicles and the link to disease biomarkers now is a major focus area. Acoustophoresis does not affect cell viability or proliferation. To determine whether acoustophoresis had any detectable effect on the intrinsic properties of viable cancer cells, we measured cell viability at two temperatures (25.0 ± 0.5°C and 37.0 ± 0.5°C) subsequent to acoustophoretic processing. cancer cells and A498 renal carcinoma cells. In cell suspensions containing viable and dead cells, we added a density modifying molecule (iodixanol) at several concentrations ranging from 0% to 30% v/v and studied the mixed cell populations during acoustophoresis in a straight channel.

Hans Lilja, Prof.